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. 2021 Dec 27;61(6):e202113784. doi: 10.1002/anie.202113784

Figure 3.

Figure 3

Compartmentalization in competitive reactions (type 2). a) Reaction Scheme for the competitive reactions of HRP and CAT following the reaction with GOx. b) Fluorescence intensity of reaction product resorufin in pathway 1 in the presence of different ratios of HRP@NR and CAT@NR. Control: experiment without HRP@NR and CAT@NR. c) Scheme of the selective compartmentalization of enzymes. System 1: GOx and HRP were co‐loaded; CAT was loaded separately. System 2: HRP was loaded separately; GOx and CAT were co‐loaded. d) Fluorescence intensity of reaction product resorufin from pathway 1 for system 1 and 2. A model system containing separately loaded GOx and HRP, in the absence of CAT (no competition), was used as reference (considered as 100 % of reaction via pathway 1). e) The selectivity of reaction to pathway 1 or 2 was calculated from the data shown in Figure 3 d. The reaction selectivity towards pathway 1 in system 1 was calculated by dividing the resorufin production rate of system 1 (grey line) by the control system (blue line). While the reaction selectivity towards pathway 2 in system 2 was calculated by 100 % minus the percentage obtained from dividing the resorufin production rate of system 2 (red line) by the control system (blue line). The error bars represent s.d. based on three identical measurements.