FIG. 5.

Effect of pH on the activity of P2O from T. multicolor in the presence of different electron acceptors. (A) Oxygen (approximately 250 μM, standard assay conditions); (B) 1,4-benzoquinone (1 mM, detection at 300 nm); (C) 2,6-dimethyl-1,4-benzoquinone (1 mM, detection at 332 nm); (D) methyl-benzoquinone (1 mM, detection at 324 nm); (E) tetrachloro-1,4-benzoquinone (250 μM, detection at 300 nm); (F) dichloroindophenol (250 μM, detection at 520 nm). The buffers used were 100 mM citrate (●),100 mM phosphate (□), and 100 mM borate (▴).