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Determination of the molecular mass and subunit composition of P2O from T. multicolor
| Method of determination |
Mr
|
||
|---|---|---|---|
| Enzyme | Dimer | Subunit | |
| Gel filtration on Superose 12 | 262,000 | ||
| SDS gradient gel electrophoresis | 65,000 | ||
| Cross-linking studies | 151,000 | 67,000 | |
| Electron microscopy | 278,000 | 69,600 | |
| Equilibrium ultracentrifugation | 270,000 | ||
| MALDI–time-of-flight analysis | 68,000 | ||
