FIGURE 6.

β‐arrestin2 binding is affected by the phosphorylation pattern of the CB1R C‐tail. SGIP1 enhances the association of CB1R‐β‐arrestin2 in cases the mutant receptors interact with β‐arrestin2. HEK293 cells were transiently co‐transfected with the CB1R‐YFP variants, β‐arrestin2‐Rluc, and empty vector/SGIP1‐mCherry (2:1:2 ratio). Cells were stimulated by 1 μM WIN. (a) Kinetic profile of β‐arrestin2 recruitment to CB1R in the presence/absence of SGIP1. (b) Kinetic profile of β‐arrestin2 recruitment to CB1R, CB1R_2A, and CB1R_2A + SGIP1. (c) Kinetic profile of β‐arrestin2 recruitment to CB1R and CB1R_6A in the presence/absence of SGIP1. (d) Kinetic profile of β‐arrestin2 recruitment to CB1R, CB1R_8A, and CB1R_8A + SGIP1. Data represent the mean ± SEM from three experiments of independent cell preparations performed in triplicate. *Represents p ≤ 0.05 (full statistical analysis is disclosed in Tables S6 and S7)