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. 2022 Jan 24;61(10):e202113477. doi: 10.1002/anie.202113477

Figure 3.

Figure 3

ATP‐fueled transient target sensing. a) Schematic illustration of ATP‐fueled transient sequence‐defined oligomer. b) Time‐dependent AGE (2 wt.%, 80 V, 2.5 h) for transient sequence‐defined oligomers with programmable lifetimes by fueling with i) 0.1, ii) 0.2, and iii) 0.4 mM ATP. c) Time‐dependent yield of complex 4 calculated from AGE. Lines are guides to the eye. d) Schematic illustration of ATP‐fueled transient sequence‐defined oligomer for multivalent sensing. e) Time‐dependent FI measurements corresponding to transient sensing of different targets. The shaded areas correspond to the standard deviation of duplicate measurements. Conditions: b) 37 °C, 10 μM of each DNA species, 0.92 WU T4 DNA ligase, 1 U BsaI, and varied concentration of ATP. e) 10 μM of each DNA species for the sequence‐defined oligomer, 5 μM target, and 0.1 mM ATP.