TABLE 1.
Evolution of solvent tolerance
| Strain | DMF concn (g/liter) | Characterization or no. of coloniesa
|
|
|---|---|---|---|
| Round 1 | Round 2b | ||
| E. coli | |||
| MM294(pMutD5) | 60 | Low-density lawnc | High-density lawnc |
| MM294(pMutD5) | 70 | 11 | 824 |
| MM294(pMutD5) | 80 | 0 | 4 |
| MM294(pMutD-wt) | 60 | 17 | Low-density lawnc |
| MM294(pMutD-wt) | 70 | 0 | 0 |
| E. coli | |||
| W1485(pMutD5) | 50 | High-density lawnc | High-density lawnc |
| W1485(pMutD5) | 60 | 386 | Low-density lawnc |
| W1485(pMutD5) | 70 | 0 | 6 |
| W1485(pMutD-wt) | 50 | High-density lawnc | High-density lawnc |
| W1485(pMutD-wt) | 60 | 0 | 0 |
| E. blattae | |||
| EB33429(pMutD5) | 50 | Low-density lawnc | High-density lawnc |
| EB33429(pMutD5) | 60 | 0 | 968 |
| EB33429(pMutD-wt) | 50 | 793 | High-density lawnc |
| EB33429(pMutD-wt) | 60 | 0 | 0 |
a
For each experiment, 106 cells were plated on LB agar containing the DMF concentration indicated.
b
Ten clones from round 1 were selected and mixed, and then 106 cells from this pool were plated on fresh LB agar plates supplemented with various concentrations of DMF.
c
Cultures that resulted in uniform growth on the plates were characterized as a high-density lawn if growth was similar to that of the no-DMF control and as a low-density lawn if growth was significantly weaker than that of the no-DMF control.