TABLE 1.
Strain | DMF concn (g/liter) | Characterization or no. of coloniesa
|
|
---|---|---|---|
Round 1 | Round 2b | ||
E. coli | |||
MM294(pMutD5) | 60 | Low-density lawnc | High-density lawnc |
MM294(pMutD5) | 70 | 11 | 824 |
MM294(pMutD5) | 80 | 0 | 4 |
MM294(pMutD-wt) | 60 | 17 | Low-density lawnc |
MM294(pMutD-wt) | 70 | 0 | 0 |
E. coli | |||
W1485(pMutD5) | 50 | High-density lawnc | High-density lawnc |
W1485(pMutD5) | 60 | 386 | Low-density lawnc |
W1485(pMutD5) | 70 | 0 | 6 |
W1485(pMutD-wt) | 50 | High-density lawnc | High-density lawnc |
W1485(pMutD-wt) | 60 | 0 | 0 |
E. blattae | |||
EB33429(pMutD5) | 50 | Low-density lawnc | High-density lawnc |
EB33429(pMutD5) | 60 | 0 | 968 |
EB33429(pMutD-wt) | 50 | 793 | High-density lawnc |
EB33429(pMutD-wt) | 60 | 0 | 0 |
For each experiment, 106 cells were plated on LB agar containing the DMF concentration indicated.
Ten clones from round 1 were selected and mixed, and then 106 cells from this pool were plated on fresh LB agar plates supplemented with various concentrations of DMF.
Cultures that resulted in uniform growth on the plates were characterized as a high-density lawn if growth was similar to that of the no-DMF control and as a low-density lawn if growth was significantly weaker than that of the no-DMF control.