TABLE 2.
Immunocytochemistry and Quantification Procedures
| Structure | Primary antibody | Q | NT1 | Control | NT1 (opiates) | R (95% CI) |
|---|---|---|---|---|---|---|
| SCN | AVP | TC | 11,749 ± 7,679 | 11,786 ± 3,926 | 18,700 | 1.00 (0.08 1.93) |
| VLPO | Galanin | CD | 44 ± 17 | 35 ± 16 | 15 | 0.80 (−0.20 1.81) |
| MLH | Hcrt | TC | 4,175 ± 2,639 | 41,493 ± 5,931 | 16,384 | 13.10 (7.90 18.29) |
| NBM | ChAT | CD | 5,155 ± 189 | 4,954 ± 797 | 3,713 | 0.96 (0.47 1.45) |
| PVN | CRH | TC | 2,862 ± 1,704 | 21,197 ± 5,517 | 0 | 9.02 (2.50 15.54) |
| PVN/SON | AVP | CD |
PVN (70 ± 30) SON (96 ± 20) |
PVN (49 ± 11) SON (127 ± 27) |
PVN (86) SON (137) |
1.34 (0.30 2.37) 1.16 (0.46 1.85) |
| PVN/SON | Oxy | CD |
PVN (34 ± 4) SON (0.056 ± 0.05) |
PVN (48 ± 10) SON (0.03 ± 0.01) |
PVN (16) SON (0.02) |
2.75 (0.51 5.00) 0.46 (−0.12 1.04) |
| PVN | Th | CD | 14 ± 6 | 27 ± 9 | 4 | 1.89 (0.22 3.56) |
| TRH | CD | 0.005 ± 0.008 | 0.014 ± 0.012 | 0.0000 | 3.33 (0.38 6.29) | |
| PVN | SCG | CD | 2 ± 2 | 15 ± 12 | 0.010 | 6.12 (−8.11 20.35) |
| PVN | PC1/3 | CD | 7 ± 3 | 14 ± 9 | 0.002 | 2.10 (−1.53 5.73) |
| IFN | NPY | CD | 10 ± 3 | 16 ± 6 | 35 | 1.59 (0.03 3.15) |
| IFN | POMC | CD | 16 ± 6 | 28 ± 11 | 40 | 2.01 (−0.25 4.27) |
| PVN | 11bHSD1 | IOD | 0.03 ± 0.001 | 0.003 ± 0.001 | 0.003 | 1.11 (−0.28 2.50) |
| PVN | HLA | IOD | 0.026 ± 0.006 | 0.030 ± 0.020 | 0.015 | 1.01 (−0.86 2.88) |
| PVN | IBA1 | IOD | 0.010 ± 0.003 | 0.010 ± 0.004 | 0.018 | 1.00 (−0.11 2.11) |
Data of the groups are expressed as mean ± standard error of the mean (SEM).
11bHSD1 = 11β‐hydroxysteroid‐dehydrogenase; AVP = arginine vasopressin; ChAT = choline acetyltransferase; CD = cell density (cell/mm2); CRH = corticotropin‐releasing hormone; Hcrt = hypocretin‐1 /orexin A; HLA = human leukocyte antigen‐DP, DQ, DR; IBA1 = Ionized calcium binding adaptor molecule 1; IFN = infundibular nucleus; IOD = integrated optical density; NBM = nucleus basalis of Meynert; NPY = neuropeptide Y; NT1 = narcolepsy type 1 (narcolepsy with cataplexy); NT1 with opiates = NBB2010‐064; Oxy = oxytocin; PC1/3 = prohormone convertase 1/3 double stain with CRH; POMC = proopiomelanocortin; POMC = proopiomelanocortin; PVN = paraventricular nucleus; Q = quantification; SCN = suprachiasmatic nucleus; SCG = secretagogin double stain with CRH; SON = supraoptic nucleus; TC = total cell number; Th = tyrosine hydroxylase; TRH = thyrotropin releasing hormone; MLH = medial lateral hypothalamus; VLPO = ventrolateral preoptic nucleus; R = mean of ratio (controls/NT1 mean); 95% CI = 95% confidence interval (lower bound upper bound).