Figure 4.
Impact of sex and aging on systolic blood pressure and intracarotid PWV in global GPER knockout mice. Main effects from two-way ANOVA are listed in the legend and results of Sidak’s multiple comparison tests are indicated on each graph. n = 8–13 mice/group. A: systolic blood pressure (BP) measured by tail cuff was not different across sex or age. B: PWV was similar in adult mice but was significantly greater in male middle-aged mice. Pint = 0.09, Page < 0.0001, Psex = 0.0001. C: total body weight was not different in adults and was increased by age in both male and female mice. Pint < 0.0001, Page < 0.0001, Psex < 0.0001. D: tibia length was not impacted by sex or age. P < 0.05. E: normalized heart weight was increased by aging in both male and female mice, and a sex difference was seen in both age groups. Pint = 0.90, Page < 0.0001, Psex = 0.0002. F: normalized kidney weights were significantly higher in male mice of both age groups. Pint = 0.17, Page = 0.0001, Psex < 0.0001. PWV, pulse wave velocity.