Figure 5.
Impact of sex and aging on passive carotid properties in global GPER KO mice. Main effects from two-way ANOVA are listed in the legend and results of Sidak’s multiple comparison tests are indicated on each graph. n = 6 to 7 mice/group. A: distensibility was greater in female mice in adulthood. Pint < 0.0001, Page < 0.0001, Psex < 0.0001. B: greater female distensibility was maintained in middle age. Pint < 0.0001, Page < 0.0001, Psex = 0.006. C: wall thickness was greater in male KO mice. Pint = 0.78, Page < 0.0001, Psex = 0.03. D: impact of sex on wall thickness was absent in middle age. Pint = 0.002, Page < 0.0001, Psex = 0.92. E and F: stress-strain curves of male and female mice were overlapping in adulthood (E), but a sex effect emerged during aging (F). G: unloaded wall thickness was not impacted by sex or aging in GPER KO mice. P > 0.05. H: unloaded lumen diameter was decreased in aging males. Pint = 0.08, Page = 0.07, Psex = 0.62. KO, knockout.