FIG. 2.

Southern blot hybridization of genomic DNA of Bradyrhizobium sp. strain IRBG271 (A) and Rhizobium sp. strain IRBG74 (B) for detection of the 16S rDNA copy number. Hybridization was performed with a homologous 16S rDNA gene probe (A) or a universal oligonucleotide probe (926f) (B). Each lane was loaded with 3 μg of DNA digested with BamHI (A, lane 1), EcoRI (A, lane 2), SmaI (A, lane 3; B, lane 2), Asp718 (A, lane 4), PstI (A, lane 5, and B, lane 1), PvuII (B, lane 3), or RsrII (B, lane 4).