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Nested IGS-targeted PCR for detection of Bradyrhizobium sp. strain IRBG271 (A) or Rhizobium sp. strain IRBG74 (B) in association with inoculated rice roots grown in unsterilized soil. The plants were grown in unsterilized rice field soil from France inoculated with different bacterial strains, and total DNA was extracted after 15 days. For the PCR conditions and sample loading, see the legend to Fig. 5.
