Figure 1.

Nuclear localization and rhythmic cycling of GFP‐ODO1 protein. Corollas of pODO1:GFP‐ODO1 line 10 (T2) and WT petals were transiently transformed with 35S:NLS‐mCherry. At 9:00 h in the morning, seven hours after end of dark, and at 17:00 h, one hour before dark, pictures were taken from the adaxial and abaxial site of corollas with a confocal microscope. mCherry signal (585‐615nm emission band) is in red, GFP signal and autofluorescence in the 505‐550nm band pass emission filter is in green, while overlapping mCherry and GFP signal appears yellow/orange. Abbreviations: GFP: GREEN FLUORESCENT PROTEIN, ODO1: ODORANT1; pODO1, 1.9 kbp promoter of ODO1; NLS, nuclear localization signal; WT, wild type.