(A) Effect of time on the yield of NZ-NO under conditions A, B, and C. (B) Nitrosation of NZ under different conditions: (I) control experiment (SGJ contains 10 mM NZ without NaNO2), (II) condition B (NZ (10 mM) in SGJ containing 40 mM NaNO2), (III) NZ (10 mM) in SGJ containing 10mM NaNO2, (IV) NZ (10 mM) in SGJ containing 5 mM NaNO2 and (V) NZ (10 mM) in SGJ containing 40 mM NaNO2 and 40 mM ascorbic acid. Each result in these experiments is the mean of three separate determination and error bars represent the standard deviation (when applicable since 100% nitrosation is associated with complete disappearance of the peak in all experiments).