Table 1.

Linearity, accuracy, and precision data for NZ and NZ-NO by the proposed method.

Linearity results
Parameter			Results
			NZ		NZ-NO
Concentration range (μg/mL)			0.1–2.0		0.5–15
Limit of detection (LOD) (μg/mL)			0.02		0.1
Limit of quantification (LOQ) (μg/mL)			0.1		0.5
Correlation coefficient (r)			0.9998		0.9999
Slope			21.32 × 103		5.41 × 103
Intercept			3.38 × 103		1.63 × 103
Standard deviation of the residuals (Sy/x)			0.29 × 103		0.16 × 103
Standard deviation of the intercept (Sa)			0.14 × 103		0.05 × 103
Standard deviation of the slope (Sb)			0.17 × 103		0.01 × 103
% RSD			1.54		1.37
% Error (% RSD/√n)			0.61		0.52
Accuracy and precision results
Analyte	Conc. (μg/mL)	Intra-day (n = 5)		Inter-day (n = 5)
		Accuracy (%)	Precision (% RSD)	Accuracy (%)	Precision (%RSD)
NZ	0.1	−1.06	2.36	−3.61	3.91
	0.6	−2.35	2.13	−3.04	2.50
	1.5	+0.11	1.84	+1.65	2.56
NZ-NO	0.5	+0.68	2.93	+1.39	3.90
	3.5	+3.38	2.00	+2.01	3.96
	15	+1.52	1.45	+3.71	2.20