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. 2019 Jun 26;27(4):906–914. doi: 10.1016/j.jfda.2019.06.001

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© 2019 Taiwan Food and Drug Administration

This is an open access article under the CC-BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 1 — Optimization results of (A) extraction method. (PPT: Protein precipitation, OE: Organic extraction, LLE: Liquid–liquid extraction) (B) extraction solvent ratio with a total volume of 300 μL and (C) extraction solvent volume with an organic solvent ratio of acetonitrile: methanol (1:2).