FIGURE 3.

Decreased tau‐seeded tau pathology in absence of NLRP3 in tau mice. (a) Schematic representation of the in vivo tau seeding protocol. Tau seeds were injected unilaterally in the CA1 region of the hippocampus and frontal cortex of tau.NLRP3−/− (T+.N−/−) and tau.NLRP3+/+ (T+.N+/+) mice at 3.5 months of age and were sacrificed at 8 months of age (4.5 months post‐injection). (b) Representative images of AT8 (anti‐tau P‐S202/T205, left panels) and AT100 (anti‐tau P‐T212/S214, right panels) immunolabeling of the ipsilateral hippocampus showing significantly decreased levels of tau phosphorylation in the CA1 region of tau‐seeded T+.N−/− (lower panels) versus tau‐seeded T+.N+/+ (upper panels) mice. (c) Higher magnification of the CA1 region (corresponding to the white squared boxes in b) are shown. (d) Quantitative analysis showed significantly decreased AT8‐ and AT100‐stained area in tau‐seeded T+.N−/− versus tau‐seeded T+.N+/+ mice. Data are shown as mean ± SEM (T+.N+/+: n = 7; T+.N−/−: n = 9; **p < .01, ***p < .001; unpaired Welch's t‐test: AT8, bar = 250 μm; AT100, bar = 100 μm)