Fig.7.

Assessment of the effects of 11P2 on MMP20-mediated amelogenin hydrolysis. A) After two hours of MMP20 hydrolysis, SDS-PAGE analysis shows that only a small amount of rH174 was hydrolyzed by MMP20, while about 50% of rH174-P2 was hydrolyzed resulting in a clear band at about 22 kDa. B) LC-MS/MS analysis shows that after incubation with MMP20, the peak of remaining rH174 reduced 15% compared to that of initial peak of rH174. However, the peak of remaining rH174-P2 was about 50% of initial rH174-P2’s. C) Turbidity analysis shows that rH714 in 50 mM Tris-HCl/10 mM CaCl₂/150 mM NaCl solution was prone to self-assemble and form supramolecules that increase the turbidity of solution. MMP20 hydrolysis enhanced the aggregation of supramolecules. rH174-P2 adapted different conformation, which had reduced propensity to self-assemble to form large size supramolecules with and without MMP20 hydrolysis.