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. Author manuscript; available in PMC: 2022 Jul 22.
Published in final edited form as: J Med Chem. 2021 Dec 13;64(24):18158–18174. doi: 10.1021/acs.jmedchem.1c01671

Figure 5. VD2173 and ZFH7116 prevent proteolytic processing of pro-HGF by matriptase, hepsin, or HGFA.

Figure 5.

Recombinant pro-HGF (10 ng) was incubated for one hour with matriptase (1 nM), hepsin (10 nM), or HGFA (5 nM) in the absence or presence of VD2173 or ZFH7116 as indicated. Immunoblotting was performed using a polyclonal antibody that recognizes pro-HGF as well as the alpha and beta chains of active HGF. Active HGF was run as a positive control.