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. Author manuscript; available in PMC: 2022 Jul 22.
Published in final edited form as: Gene Ther. 2007 Feb 1;14(7):584–594. doi: 10.1038/sj.gt.3302897

Figure 2.

Figure 2

Lentiviral vector-mediated expression of MYO7A. (a–c) Immunofluorescence shows MYO7A immunolabel in (a) Myo7a+/− RPE, (b) Myo7a−/− RPE, 7 days after infection with LV-MYO7A(B), and (c) Myo7a−/− RPE, 7 days after infection with LV-MYO7A(A). (d) A phase contrast image of (c). (e) Western blot of MYO7A protein (upper panel) in Myo7a+/− RPE (Ctrl), Myo7a−/− RPE (Mut) and Myo7a−/− RPE, 5 days after infection with LV-MYO7A(B) (Mut+B). HSP60 labeling (lower panel) was used as a loading control. (f) Alkaline phosphatase (AP) histochemistry of a retinal section from an albino control mice. The retina was injected at P4 with LV-AP(B) and fixed at P14. Arrows and arrowheads indicate AP staining in the RPE and photoreceptor cells, respectively. Area shown is away from the site of injection. (g, h) MYO7A immunostaining (arrows) of the RPE in the central (g) and peripheral (h) regions of the retina from an albino Myo7a−/− mouse. The retina was injected centrally at P96 with the LV-MYO7A(B) virus and fixed 6 days later. Faint, non-specific staining9 is evident in the photoreceptor synaptic layer. RPE, retinal pigment epithelium; ONL, outer nuclear layer. Scale bars = 20 μm (a–d), 50 μm (f–h).