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. 2022 Jul 22;8(29):eabn3773. doi: 10.1126/sciadv.abn3773

Fig. 5. Impact of liraglutide treatment on islet protein secretion.

Fig. 5.

(A) In vitro study paradigm for generation of conditioned media samples used for proteomics analysis. Conditioned media generated from islets of liraglutide- (LIRA) and saline-treated (CTRL) β cell GLP-1R WT mice were digested, and protein signatures were determined using mass spectrometry–based quantitative proteomics. LFQ, label-free quantitation. (B) Quantitative data were filtered using SignalP and literature searches to identify 67 of the total 191 proteins as secreted proteins. (C) Score plot of OPLS-DA showing the cluster separation between CTRL and LIRA-conditioned media samples. (D) Volcano plot of differentially expressed proteins for CTRL versus LIRA- conditioned media. Significantly (P < 0.05) and >2-fold differentially expressed proteins are displayed in green. Significantly differentially expressed proteins identified as secreted proteins are displayed in blue. n = 3 per group. False discovery rate < 0.05.