Fig. 5. Knocking out of Ezh2 caused lineage infidelity of incisor epithelium and thus impaired enamel matrix production capability during regeneration.

(A) Immunofluorescent images of Sox2 at d5 after clipping. (B) Immunofluorescent images of Ki67 at d5 after clipping. (C) Statistical analysis on the number of Sox2⁺ or Ki67⁺ cells in LaCL region of (A) and (B). (D) Statistical analysis on the ratio of aberrant emergence in (A) and (B). (E) Double-staining immunofluorescent images of Sox2 and Amelx in secretory ameloblast region at d5 after clipping. (F) f1: Double-staining immunofluorescent images of tdTomato and Amelx at d5 after clipping. f2: Statistical analysis on tdTomato⁺Amelx⁺ area in f1. (G) g1: Masson’s trichrome images at d5 after clipping. The arrows in the high magnification indicated enamel matrix, which was dyed red. g2: Statistical analysis on the location of the onset points of enamel matrix in g1. g3: Statistical analysis on the area of enamel matrix in g1. All representative images and data (means ± SEM) were generated from at least six slices from six mice for each group. ***P < 0.001. The dot-square contoured regions were shown in the right as high-magnification images. Scale bar, 50 μm.