Author response image 2. Western blot (A: Tris‐acetate gel; B: Tris‐glycine gel) using membrane preparations from tsA201 cells transiently transfected with Cav2.3e α1, β3/β2a and α2δ1 or an empty vector (pUC) and mouse whole brain protein preparationsas described by us previously (1, 2).

Primary antibody: mouse anti‐β2 (A: 1:1,000; B: 1:800; Antibodies Inc, Cat# 75‐065), secondary antibody: goat anti‐mouse IgG (A: 1:5,000; B: 1:3,000; Invitrogen, Cat# 31430). M: Marker PageRuler Plus Prestained Protein Ladder (Thermo Scientific, Cat# 26619); M2: Marker Spectra Multicolor High Range Protein Ladder (Thermo Scientific, Cat# 26625).