| Mouse strain:C57Bl/6N, C57Bl/6J |
C57Bl/6N, C57Bl/6J |
Charles River |
C57Bl/6N, C57Bl/6J |
|
| Mouse strain:C57Bl/6-TH-GFP mice |
C57Bl/6-TH-GFP |
Matsushita et al., 2002; Sawamoto et al., 2001
|
|
GFP-expression under TH-promotor |
| Transfected construct |
Human Cav1.3L α1-subunit (C-terminally long Cav1.3 splice variant) |
Koschak et al., 2001
|
GenBank accession number EU363339
|
Cloned into proprietary vector lacking GFP ("GFPminus"), described in Grabner et al., 1998
|
| Transfected construct |
Human Cav2.3 α1-subunit |
Pereverzev et al., 2002
|
|
Cloned into pcDNA3 vector |
| Transfected construct |
Rat β3-subunit |
Koschak et al., 2001
|
GenBank: NM_012828, |
Cloned into pCMV6 vector |
| Transfected construct |
Rat β2a-subunit |
Koschak et al., 2001
|
GenBank: M80545
|
Cloned into pCMV6 vector |
| Transfected construct |
Mouse β2d-subunit |
β2aN1, Link et al., 2009
|
GenBank: FM872408.1
|
Cloned into pCAGGS vector |
| Transfected construct |
Mouse β2e-subunit |
β2aN5, Link et al., 2009
|
GenBank: FM872407
|
Cloned into pCAGGS vector |
| Transfected construct |
C3S/C4Sβ2a |
Cysteine residues in position 3 and 4 of β2a replaced by serines, Gebhart et al., 2010
|
|
Cloned into pCMV6 vector |
| Transfected construct |
Rat β4-subunit (splice variant β4e) |
Etemad et al., 2014
|
|
Cloned into pβA vector |
| Cell line (Homo sapiens) |
TsA-201 cells |
European Collection of Authenticated Cell Cultures |
ECACC, catalogue # 96121229, lot # 13D034 |
Tested Mycoplasma negative |
| Antibody |
Anti-tyrosine hydroxylase (TH; rabbit polyclonal) |
Merck Millipore |
Cat#: 657012, |
1:1000 |
| Commercial assay or kit |
RNeasy Lipid Tissue Mini Kit |
Qiagen GmbH, Germany |
catalog # 1023539 |
|
| Commercial assay or kit |
Phenol/guanidine-based Qiazol lysis reagent |
Qiagen GmbH, Germany, |
catalog # 79,306 |
|
| Commercial assay or kit |
Maxima H Minus First Strand cDNA synthesis kit |
Thermo Fisher Scientific, Waltham, MA, USA |
catalog # K1682 |
|
| Commercial assay or kit |
Quant-IT PicoGreen dsDNA Assay Kit |
Thermo Fisher Scientific, Waltham, MA, USA |
catalog # P7589 |
|
| Commercial assay or kit |
Mycoplasma Detection Kit |
ATCC |
Universal Mycoplasma Detection Kit 30–1012 K |
|
| Commercial assay or kit |
TaqMan Universal PCR Master Mix |
Thermo Fisher Scientific, Waltham, MA, USA |
|
|
| Commercial assay or kit |
RNAscope technology |
Advanced Cell Diagnostics (ACD) |
RNAscope Fluorescent Multiplex Detection Kit (ACD, Cat# 320851) |
|
| Chemical compound |
Neurobiotin-labeling reagent: Streptavidin Alexa Fluor conjugate 647 |
Thermo Fisher Scientific, Waltham, MA, USA |
catalog # S21374
|
1:1000 |
| Chemical compound |
SNX-482 |
Alomone Labs, Jerusalem, Israel |
catalog # RTS-500 |
|
| Software, algorithm |
Clampfit |
Clampfit |
Version 10.0 or 10.7, RRID:SCR_011323
|
|
| Software, algorithm |
QuantStudioTM |
Thermo Fisher Scientific, Waltham, MA, USA |
Quantstudio 3 Software |
|
| Software, algorithm |
Fiji |
https://imagej.net/Fiji, Schindelin et al., 2012
|
|
|
| Software, algorithm |
GraphPad Prism |
GraphPad |
GraphPad Prism Ver 5, 7.04 and 8 (RRID:SCR_002798) |
|
| Software, algorithm |
SigmaPlot |
Systat Software, Germany |
SigmaPlot Ver 14, RRID:SCR_003210
|
|
