Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (Homo sapiens) | HEK293 cells | ATCC | CRL-1573; RRID: CVCL_0045 | Human embryonic kidney (female) |
| Cell line (Homo sapiens) | A3.01-R5 | Colin et al., 2013 | CEM T cell line derivated cells | |
| Antibody | α-GFP (mouse monoclonal) |
Roche | 11814460001 | Flow cytometry dilution (1: 100) |
| Antibody | α-CCR5 2D7 (mouse monoclonal) |
BD-Biosciences | 555,991 | Flow cytometry dilution (1: 500) |
| Antibody | FLAG tag M2 (mouse monoclonal) |
Sigma | Cat# F3165 | Flow cytometry dilution (1: 750) |
| Antibody | FLAG tag M2-Cy3 (mouse monoclonal) |
Sigma | Cat# A9594 | TIRF microscopy dilution (1: 1000) |
| Antibody | Phospho ERK1/2 (mouse monoclonal) | Cell signaling | Cat# 9,106 | Western blot dilution (1:2500) |
| Antibody | ERK2 (Rabbit polyclonal) | Santa-Cruz Biotech |
Cat# sc-154 | Western blot dilution (1:750) |
| Antibody | Goat anti-mouse HRP (rat monoclonal) | BD-Biosciences | Cat# 559,751 | Western blot dilution (1:120000) |
| Antibody | Goat anti-rabbit HRP (goat polyclonal) | Jackson | Cat# 111-035-144 | Western blot dilution (1:3500) |
| Antibody | Goat anti-mouse phycoerythrin (PE) (goat polyclonal) | BD-Biosciences | Cat# 550,589 | Flow cytometry dilution (1:100) |
| Recombinant DNA reagent | pmCherry- (plasmid) |
other | Provided by F. Perez (Institut Curie). | |
| Recombinant DNA reagent | peGFP-CCR5 (plasmid) |
other | Provided by F. Perez (Institut Curie). | |
| Recombinant DNA reagent | peGFP-CCR5-L196K (plasmid) |
This paper | Contains a point mutation in CCR5 at position L196. | |
| Recombinant DNA reagent | pFLAG-SNAP-CCR5-WT (plasmid) |
Jin et al., 2018 | Provided by Cisbio | |
| Recombinant DNA reagent | pFLAG-SNAP-CCR5-L196K (plasmid) |
Jin et al., 2018 | Introduction of a lysine in position L196 | |
| Recombinant DNA reagent | pβarr2-GFP | Storez et al., 2005 | Provided by S. Marullo (Institut Cochin) | |
| SiRNA reagent | βarr1/2 (siRNA) |
Dharmacon | See Materials and methods for sequence | |
| SiRNA reagent | Scrambled (siRNA) | Dharmacon | See Materials and methods for sequence | |
| Soluble protein | HIV-1 gp120 #25, #34 |
Colin et al., 2018 | Gp120 from PBMCs of patients in early or late HIV-1 infection stage. See details in ‘cell culture and reagents’ section of 'Materials and methods' | |
| Soluble protein | Human sCD4 | Colin et al., 2018 | See details in ‘cell culture and reagents’ section of 'Materials and methods' | |
| Chemical compound, drug | Maraviroc | NIH | Cat# ARP-11580 | CCR5 inverse agonist |
| Chemical compound, chemokine | CCL4 | This paper | Provided by F. Baleux (Institut Pasteur) | |
| Chemical compound, drug | PSC-RANTES | NIBSC | Cat# ARP973 | CCR5 agonist |
| Chemical compound, chemokine | SDF-1 | Peprotec | Cat# 300–28 A | CXCR4 agonist |
| Chemical compound, drug | Pertussis Toxin | Sigma | Cat#179 A | 100 ng/ml |
| Software, algorithm | Prism | GraphPad | 8.1.1 | |
| Software, algorithm | ICY | Open access | Version 2.4.0.0 | https://icy.bioimageanalysis.org/ |
| Software, algorithm | MATLAB | MathWorks | R2017a |