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An acentriolar AKAP450 knockout RPE1 cell stably expressing GFP-CDK5RAP2 (green) and labeled overnight with 100 nM SiR-tubulin (red) was imaged for ~7 min (200 frames, 2 s interval) prior to treatment with 10 μM nocodazole. Nocodazole was washed out at ~27 min (frame 801) when all microtubules were depolymerized. Time is min: s.
