Figure 4.

C+P reduced protein levels of NOX subunits after OGD/R in SHSY5Y cells. (a) gp91^phox, (b) p67^phox, (c) p47^phox, and (d) p22^phox, identified by Western blot at 6 and 24 hours of reoxygenation. Levels of gp91^phox and p67^phox protein increased after OGD but decreased after C+P therapy at 24 hours of reoxygenation. Moreover, p47^phox levels increased after OGD, but C+P lowered this expression at 6 and 24 hours of reoxygenation. OGD induced the elevation of p22^phox, and there was a greater reduction in p22^phox expression in the OGD/C+P treatment group versus the OGD group at 6 hours of reoxygenation. ^∗p < 0.05 versus the OGD group; ^#p < 0.05, ^##p < 0.01 versus the OGD/C+P group. Results are shown as mean ± SE of three independent experiments. The representative immunoblots are presented.