TABLE 1.

Purification of V. tubiashii cytolysin

Stage	Vol (ml)	Amt of protein (mg)	Activity (HU)	Sp act (HU/mg)	Recovery (%)
(1) Culture supernatant fluids	430	9.89	21,500	2,174	100
(2) Hydrophobic interaction chromatography	19.3	0.87	9,650	11,092	45
(3) Gel filtration chromatography	55	0.041	6,875	167,683	32