TABLE 2.

Inactivation of V. tubiashii cytolysin

Treatment	Residual activity (%)
27°C	100
37°C (PBS)	50
37°C (PBS-BSA)	100
56°C (PBS)	0
56°C (PBS-BSA)	6
100°C	0
Control (PBS, 37°C for 30 min)	50
Papain, 50 μg	50
Trypsin, 50 μg	50
Chymotrypsin, 50 μg	4
Subtilisin, 50 μg	4
Control (PBS, 25°C for 30 min)	100
Cholesterol (μg)
0.1	15
1	8
10, 25, 50, and 100	0