Figure 7.

Chronic variable stress (CVS) but not chronic repeated restraint stress (CRS) increases anxiety-like behavior

(A and B) At the end of chronic stress protocols, open field (OF) and elevated plus maze (EPM) tests were performed. In the OF test, reduced centrum preference (A) and latency of the first entrance into the corner (B) were recorded in CVS exposed mice, while these parameters were unchanged in CRS exposed mice.

(C and D) Representative trajectory diagrams for control (blue) and chronically stressed (CRS and CVS) mice are shown on (C). Reduced open arm preference of CVS exposed mice in EPM test is demonstrated on (D).

Unpaired t-test was used to calculate significance. Data are presented as mean ± SEM. ∗p < 0.05 vs. the control group.