Figure 8.

Acute- and chronic stress exposure differentially affects expression of neuronal activation markers in the hypothalamus

Hypothalamic mRNA levels of Fos, Fosl2 and Fosb were measured by RT qPCR. Fold change values, relative to controls, are shown in acutely restrained animals and mice exposed to repeated restraint (CRS) or chronic unpredictable variable (CVS) stress. Unpaired t-test was used to calculate significance. Data are presented as mean ± SEM data. ∗p < 0.05; ∗∗∗p < 0.001; ∗∗∗∗p < 0.0001 vs. control group. ^##p < 0.01; ^#####p < 0.0001 vs. acute stress.