| Chemicals, peptides, and recombinant proteins |
|
| EvaGreen® Dye, 20X in Water |
Biotium |
Cat# 31000 |
| LightCycler 480 Probes Master |
Roche |
Cat# 04887301001 |
|
| Critical commercial assays |
|
| Corticosterone RIA kit |
Institute of Isotopes Budapest, Hungary |
Cat# RK-240CT |
| Glucose Colorimetric Detection Kit |
Invitrogen |
Cat# EIAGLUC |
| High-Capacity cDNA Reverse Transcription Kit |
ThermoFisher Scientific |
Cat# 4368814 |
| Multi-Care-in; Triglyceride |
Biochemical Systems International S.p.A. |
Cat# BC10-MULTICAREIN |
| Total RNA Mini Kit (tissue) |
Central European Biosystems GmbH |
Cat# RT100 |
|
| Experimental models: Organisms/strains |
|
| Mouse: C57BL/6J |
The Jackson Laboratories |
RRID:IMSR_JAX:000664 |
|
| Oligonucleotides |
|
|
Gapdh qPCR target sequence forward: 5′-TGACGTGCCGCCTGGAGAAA-3′ reverse: 5′-AGTGTAGCCCAAGATGCCCTTCAG-3′ |
Microsynth AG (custom order) |
N/A |
|
Fos qPCR target sequence forward: 5′-GAGAGCCTTTCCTACTACCATTCC-3′ reverse: 5′-GGACAGATCTGCGCAAAAGTC-3′ |
Microsynth AG (custom order) |
N/A |
|
Fosl2 qPCR target sequence forward: 5′-CACCGCGGATCATGTACCAG-3′ reverse: 5′-TATCTACCCGGAACTTCTGCTG-3′ |
Microsynth AG (custom order) |
N/A |
|
FosB qPCR target sequence forward: 5′-CAGATCGACTTCAGGCGGAAAC-3′ reverse: 5′-AATCTCTCACCTCGGCCAGC-3′ |
Microsynth AG (custom order) |
N/A |
|
| Software and algorithms |
|
| EthoVision XT |
Noldus |
https://www.noldus.com/ethovision-xt |
| Prism 6 |
GraphPad |
https://www.graphpad.com/ |
| StepOne™ Software v2.3 |
Applied Biosystems |
https://www.thermofisher.com/ |