Figure 2.

Morphology of iPSC cells transition to RIM phase: Day 2 to day 1

(A and B) Differentiating cells at day-1 (A) and day 0 (B). The cells at day-1 are spiky because of the blebbistatin in culture media. The images represent good density and morphology of differentiating cells.

(C and D) Differentiating cells at day 0. The arrowheads represent the abnormal morphology that should be avoided in day 0 cells. The probable reason for such morphologies is the excessive use of TrypLE and mechanical force during passaging.

Scale bar, 200 μm.