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Morphology of differentiating cells in RMNA phase: Day 11 to day 20
(A and B) Differentiating cells at day 16 (A) and day 20 (B). At day 20, appearance of little bumps of neuronal clusters (arrowhead marked) indicates successful run. The flat land around bumps RPE progenitor cells.
(C and D) Images of bad cultures at day 16 (A) and day 20 (B). The opaque cultures lacking any islands of neuronal islands end up dying at the end of day 20, i.e., RMNA phase.
Scale bar, 20 μm.
