Surface type	Volume	VTN concentration	Vol/surface area
6-well plate	2 mL/well	2.5 μg/mL	200 μL/cm2
T-75 flask	20 mL	2.5 μg/mL
T-150 flask	30 mL	2.5 μg/mL
12 well trans well plate	0.5 mL on apical side	2.5 μg/mL