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Retinal Induction Media (RIM)
| Reagent | Final concentration | Amount |
|---|---|---|
| DMEM/F12 | N/A | 500 mL |
| CTS™ KnockOut™ SR XenoFree Or Knockout KSR |
1.5% | 7.5 mL |
| MEM non-essential AA | 0.1 mM | 5 mL |
| Sodium pyruvate | 1.0 mM | 5 mL |
| CTS™ N-2 supplement or N-2 supplement | 1.0% | 5 mL |
| B-27® supplement (+VitA) | 2.0% | 10 mL |
| Ascorbic acid | 200 μM | 500 μL |
| LDN-193189 | 10 nM | 500 μL |
| SB 431542 | 1.0 μM | 12.5 μL |
| CKI-7 Dihydrochloride | 0.5 μM | 50 μL |
| AF-IGF-1 | 1 ng/mL | 5 μL |
| Total | NA | 500 mL |
