Reagent | Final concentration | Amount |
---|---|---|
MEM alpha | N/A | 500 mL |
Heat inactivated fetal bovine serum | 5% | 25 mL |
N-2 supplement | 1% | 5 mL |
MEM non-essential amino acids | 0.1 mM | 5 mL |
Sodium pyruvate | 1 mM | 5 mL |
Penicillin-Strep | 1× | 5 mL |
Taurine | 250 μg/mL | 2.5 mL |
Hydrocortisone | 20 μg/L | 553 μL |
T3 | 0.013 μg/L | 330 μL |
Total | NA | 500 mL |