Reagent | Final concentration | Amount |
---|---|---|
DPBS (without Ca2+ and Mg2+) | N/A | 1000 mL |
Heat inactivated fetal bovine serum | 2% | 20 mL |
Ultra-Pure EDTA solution | 2 mM | 4 mL |
Total | NA | 1000 mL |
Reagent | Final concentration | Amount |
---|---|---|
DPBS (without Ca2+ and Mg2+) | N/A | 1000 mL |
Heat inactivated fetal bovine serum | 2% | 20 mL |
Ultra-Pure EDTA solution | 2 mM | 4 mL |
Total | NA | 1000 mL |