Permeabilization buffer
| Reagent | Final concentration | Amount |
|---|---|---|
| DPBS (without Ca2+ and Mg2+) | 98% | 49 mL |
| Heat inactivated fetal bovine serum | 2% | 1 mL |
| Triton-X-100 | 0.2% | 0.1 mL |
| Total | NA | 50 mL |
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