| Culture surface | Seeding numbers | Media volume | Number of plates/flasks |
|---|---|---|---|
| 6 well plates | 200 K–400 K/well | 3 mL per well | 3- 6-well plates |
| T75 | 1.5–3.0 million | 15 mL | 2 T75 |
| T150 | 3.0–5.0 million | 30 mL | 1 T150 |
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