| Antibodies | First dilution | Volume (μL) of Ab needed in 50 μL of permeabilization buffer | Second Dilution |
Volume of Ab needed in 50 μL of permeabilization buffer |
|---|---|---|---|---|
| Anti-PMEL17 | 1:25 | 2 μL | 1:10 | 5 μL |
| Anti-TYRP1 | 1:25 | 2 μL | 1:10 | 5 μL |
| IgG1 (Isotype control for PMEL-17, TYRP1) | 1:25 | 2 μL | 1:25 | 2 μL |
| IgG Alexa Fluor-488 | 1:10 | 5 μL | 1:5 | 10 μL |
| Anti-PAX6 | 1:1500 | |||
| Anti-MITF | 1:200 |
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