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. 2022 Jul 20;3(3):101582. doi: 10.1016/j.xpro.2022.101582
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

PAX6 (1:1500) Covance PRB-278P
MITF (1:200) Exalpha X1405M
TYRP1 (1:250) Novus Biologicals NBP2-32901
CD24 (1:500) BD Biosciences 655154
CD56 (1:500) BD Biosciences 340723
Anti-FITC MicroBeads (1:10) Miltenyi 130-048-701
Anti-mouse Alexa-Flour-488 (1:50) Life Technologies A21202

Chemicals, peptides, and recombinant proteins

Activin-A R&D Systems 338-AC/CF
IGF-1 R&D Systems AFL291
PD0325901 R&D Systems 4192
Nicotinamide Sigma N0636
LDN-193189 Stemgent 04-0074
SB 431542 R&D Systems 1614/10
CKI-7 Dihydrochloride Sigma C0742
Blebbistatin Sigma B0560
Ascorbic Acid Sigma A4544
Rock-Inhibitor R&D Systems 1254
3,3′,5-Triiodo-L-thyronine Sigma T5516
Taurine Sigma T0625
Hydrocortisone Sigma H6909
Water for Injection (WFI) Thermo Fisher Scientific A12873-01
10 M NaOH Sigma 72068
Vitronectin Life Technologies A14701SA
DPBS Life Technologies 14190-144
CryoStor® CS10 STEMCELL
Technologies
07930
FBS Hyclone SH30071.03
KnockOut™ SR XenoFree Life Technologies 10828028
KnockOut™ SR Life Technologies 10828010
MEM Alpha Thermo Fisher Life Technologies 12571-063
DMEM/F12 Life Technologies 11330-032
MEM non-essential AA Life Technologies 11140
E8 media Life Technologies A1517001
Penicillin-Strep Life Technologies 15140-148
Sodium Pyruvate Life Technologies 11360-070
CTS N-2 Supplement Life Technologies A13707-01
N-2 Supplement Life Technologies 17502048
B-27® Supplement (+VitA) Life Technologies 17504-044
0.5 M EDTA Life Technologies 15575-020
DMSO Sigma D2650
16% Formaldehyde Electron Microscopy Sciences 50-980-487
Prostaglandin E2 R&D Systems 2296
Versene Life Technologies 15040066
TrypLE Express Life Technologies 12605028

Experimental models: Cell lines

Human: iPSC, Female, 83 years, healthy subject in house generated N/A

Other

6-well plates Nunc 140675
T-75 flasks Nunc 156499
T-150 flasks TPP 90151
96 well plates- v bottom for FLOW assay Thermo Fisher Scientific 249570
20 μm sterifilp Sigma SCNY00020
0.22 μm filter units-50 mL Sigma SCGP00525
0.22 μm filter units-250 mL Corning 431096
0.22 μm filter units-500 mL Corning 431118
LD Columns Miltenyi 130-042-901
MidiMACS or QuadroMACS separator Miltenyi 130-042-302 /130-090-976
ThawSTAR CFT BioLife BCS601