REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
PAX6 (1:1500) | Covance | PRB-278P |
MITF (1:200) | Exalpha | X1405M |
TYRP1 (1:250) | Novus Biologicals | NBP2-32901 |
CD24 (1:500) | BD Biosciences | 655154 |
CD56 (1:500) | BD Biosciences | 340723 |
Anti-FITC MicroBeads (1:10) | Miltenyi | 130-048-701 |
Anti-mouse Alexa-Flour-488 (1:50) | Life Technologies | A21202 |
Chemicals, peptides, and recombinant proteins | ||
Activin-A | R&D Systems | 338-AC/CF |
IGF-1 | R&D Systems | AFL291 |
PD0325901 | R&D Systems | 4192 |
Nicotinamide | Sigma | N0636 |
LDN-193189 | Stemgent | 04-0074 |
SB 431542 | R&D Systems | 1614/10 |
CKI-7 Dihydrochloride | Sigma | C0742 |
Blebbistatin | Sigma | B0560 |
Ascorbic Acid | Sigma | A4544 |
Rock-Inhibitor | R&D Systems | 1254 |
3,3′,5-Triiodo-L-thyronine | Sigma | T5516 |
Taurine | Sigma | T0625 |
Hydrocortisone | Sigma | H6909 |
Water for Injection (WFI) | Thermo Fisher Scientific | A12873-01 |
10 M NaOH | Sigma | 72068 |
Vitronectin | Life Technologies | A14701SA |
DPBS | Life Technologies | 14190-144 |
CryoStor® CS10 | STEMCELL Technologies |
07930 |
FBS | Hyclone | SH30071.03 |
KnockOut™ SR XenoFree | Life Technologies | 10828028 |
KnockOut™ SR | Life Technologies | 10828010 |
MEM Alpha Thermo Fisher | Life Technologies | 12571-063 |
DMEM/F12 | Life Technologies | 11330-032 |
MEM non-essential AA | Life Technologies | 11140 |
E8 media | Life Technologies | A1517001 |
Penicillin-Strep | Life Technologies | 15140-148 |
Sodium Pyruvate | Life Technologies | 11360-070 |
CTS N-2 Supplement | Life Technologies | A13707-01 |
N-2 Supplement | Life Technologies | 17502048 |
B-27® Supplement (+VitA) | Life Technologies | 17504-044 |
0.5 M EDTA | Life Technologies | 15575-020 |
DMSO | Sigma | D2650 |
16% Formaldehyde | Electron Microscopy Sciences | 50-980-487 |
Prostaglandin E2 | R&D Systems | 2296 |
Versene | Life Technologies | 15040066 |
TrypLE Express | Life Technologies | 12605028 |
Experimental models: Cell lines | ||
Human: iPSC, Female, 83 years, healthy subject | in house generated | N/A |
Other | ||
6-well plates | Nunc | 140675 |
T-75 flasks | Nunc | 156499 |
T-150 flasks | TPP | 90151 |
96 well plates- v bottom for FLOW assay | Thermo Fisher Scientific | 249570 |
20 μm sterifilp | Sigma | SCNY00020 |
0.22 μm filter units-50 mL | Sigma | SCGP00525 |
0.22 μm filter units-250 mL | Corning | 431096 |
0.22 μm filter units-500 mL | Corning | 431118 |
LD Columns | Miltenyi | 130-042-901 |
MidiMACS or QuadroMACS separator | Miltenyi | 130-042-302 /130-090-976 |
ThawSTAR CFT | BioLife | BCS601 |