RPE-MM | Stock concentration | Final concentration | Volume |
---|---|---|---|
MEM alpha | 1× | N/A | 500 mL |
Heat inactivated fetal bovine serum | 1× | 5% | 25 mL |
N-2 supplement | 100× | 1% | 5 mL |
MEM non-essential amino acids | 10 mM | 0.1 mM | 5 mL |
Sodium pyruvate | 100 mM | 1 mM | 5 mL |
Penicillin-Strep | 1× | 5 mL | |
Taurine | 50 mg/mL | 250 μg/mL | 2.5 mL |
Hydrocortisone | 18.1 mg/L | 20 μg/L | 553 μL |
T3 | 20 mg/mL | 0.013 μg/L | 330 μL |