MACS buffer | Stock concentration | Final concentration | Volume |
---|---|---|---|
DPBS (without Ca2+ and Mg2+) | 1× | N/A | 1000 mL |
Heat inactivated fetal bovine serum | 1× | 2% | 20 mL |
Ultra-Pure EDTA solution | 0.5 M | 2 mM | 4 mL |
MACS buffer | Stock concentration | Final concentration | Volume |
---|---|---|---|
DPBS (without Ca2+ and Mg2+) | 1× | N/A | 1000 mL |
Heat inactivated fetal bovine serum | 1× | 2% | 20 mL |
Ultra-Pure EDTA solution | 0.5 M | 2 mM | 4 mL |