Table 2.

Kinetics of deacylated tRNA^Lys dissociation from the E site.

EF-G	Dissociation rate from E site, s−1 (N)a,b
	U AAG AAG (non-slippery)	A AAA AAG (slippery)
wt	0.9 ± 0.1 (196)	1.0 ± 0.1 (169)
Q507D	0.6 ± 0.1 (175)	0.5 ± 0.1 (152)
wt–GTPγS	0.2 ± 0.1 (205)	0.3 ± 0.1 (173)
wt–Spc	n.d.	0.3 ± 0.1 (157)
wt–FA	<0.1 ± 0.1c (169)	n.d.

^aN, number of traces.

^bAll rates were corrected for photobleaching of the FRET dyes (Methods) and are shown as mean±s.d. from at least three independent experiments. R² ≥ 0.96 in all cases.

^cRate was limited by the photobleaching of the FRET dyes.