Figure 2.

Differentially expressed (DE) tRDFs in tumor and normal samples

(A and B) Heatmap shows the DE tRDFs in two GEO datasets; samples were pairwise by tumor and normal. Up- and downregulation of DE tRDFs could be separated by unsupervised clustering. (A) DE tRDFs in GEO: GSE83527 are shown; (B) DE tRDFs in GEO: GSE62182 are shown. (C and D) Boxplot shows the 11 tRDFs that were identified as common DE in GEO: GSE83527 and GSE62182 datasets; p value of each group is less than 0.05. (E) Correlogram shows positive (blue) and negative (red) correlation among 11 DE tRDFs as determined by the Pearson correlation analysis. (F and G) AUCs show the independent validation results of GEO: GSE1109067 (AUC: 0.914) and TCGA-LUAD (AUC: 0.905) from random forest model. (H) Boxplot shows the expression level of six DE tRDFs in TCGA-LUAD cohort that were found in common from GEO DE tRDFs. (I) AUCs show the diagnostic effect of six tRDFs signatures from five independent validations. Each validation contains different groups of tRDFs from signatures; one tRDF would be removed from previous group in order as 5P_tRNA-Asn-GTT-2-3, 5a_tRF-Asp-GTC, 3P_tRNA-Arg-TCG-1-1, 3P_tRNA-Arg-TCT-4-1, 5a_tRF-Ile-AAT/GAT, and 5P_tRNA-Gly-TCC-1-1 and finally ends as last two tRDFs.