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. 2022 Jul 22;12:12580. doi: 10.1038/s41598-022-16789-w

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Sample purity and genome coverage of single cells of Nostoc sp. CCCryo 231-06 exposed to different media/substrates and exposure conditions during the BIOMEX experiment. Number of replicates per experimental conditions are described in Table S1. Different media/substrates are color-coded. (a) The horizontal axis represents the percentage of reads that were classified to the genus Nostoc after the metagenomic analysis of each sample and replicate. The vertical axis represents the fraction of the recovered Nostoc sp. CCCryo 231-06 that is mapped by the reads of each sample and replicate. The size of the circle represents the number of reads present in each sample and replicate. The components of the mineral substrates (lunar and Martian regoliths) are provided in Table S2. (b) Average sample purity and genome coverage fraction are sorted by medium and exposure condition. Samples on BG11 displayed significantly higher purity and genome coverage. Samples exposed to UV radiation on the ISS displayed significantly higher purity than those in other experimental conditions. Student’s t test was used to calculate the p-values. BG11 blue-green medium, ISS international space station, P-MRS phyllosilicatic martian regolith, S-MRS sulfatic martian regolith, UV ultraviolet light, Ground/UV earth with UV exposure, ISS/Dark ISS without UV exposure, ISS/UV ISS with UV exposure.