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. 2022 Jul 22;12:12574. doi: 10.1038/s41598-022-13421-9

Figure 2.

Figure 2

Cinnamon extract (CE) regulates Acyl-CoA synthetase long-chain family 1 (ACSL1) mRNA and protein expression in a time-dependent manner in 3T3-L1 adipocytes. 3T3-L1 adipocytes were serum-starved for 8 h in DMEM and then treated with 30 μg/mL of CE for 0.5–16 h. (a) Real-time PCR was employed to determine ACSL1 mRNA expression. (b) The levels of ACSL1 protein expression were measured via western blotting. The image is a cropped blot and the full-length images of the western blot for the three experiments are provided in Supplementary Fig. S2a. Each value represents the mean ± SD of three different experiments (n = 3/group). *p < 0.05, compared with the control values.