Figure 4.

Triacsin C (TriC), an inhibitor of ACSL1, and Cinnamon extract (CE), regulate glucose uptake and ACC phosphorylation in 3T3-L1 adipocytes. 3T3-L1 adipocytes were serum-starved for 8 h in DMEM and then challenged with 10 µM TriC for 45 min or with 30 µg/mL of CE for 30 min. (a) ACSL1 expression levels were measured via western blotting. (b) The uptake of 2-deoxyglucose by the cells was assayed, as described in the Materials and Methods section. (c–e) The levels of AMPK, ACC, and Akt phosphorylation were measured via western blotting using total AMPK, ACC, and Akt as loading controls. Each value represents the mean ± SD of three different experiments (n = 3/group). Alphabet letters indicate intergroup comparisons. Different alphabet indicate statistically significant differences among groups (P<0.05). The image is a cropped blot and the full-length images of the western blot for the three experiments are provided in Supplementary Fig. S4 a–d. In Fig. S4d images, different parts of the same membrane were cut out and reacted with different antibodies. The cutouts are clearly distinguished using yellow separator lines.