Figure 5.

Cinnamon extract (CE) improves glucose intolerance and ACSL1 protein expression in type 2 diabetic mice. N: nondiabetic normal control group. Mice were orally administered 1-mL pure water for 8 weeks. NCE: CE-treated nondiabetic normal control group. CE (100 mg/kg bw/day) was orally administered for 8 weeks. 2DM: type 2 diabetes model mice group. Mice were orally administered 1-mL pure water for 8 weeks. 2DMCE: CE-treated type 2 diabetes model mice group. CE (100 mg/kg bw/day) was orally administered for 8 weeks. (a) For the oral glucose tolerance test, the mice fasted for 6 h prior to the test. Glucose (1.5 g/kg bw) was orally administered, and the blood glucose concentration was measured using the blood obtained via the tail vein at the indicated time points after the oral glucose loading. (b) The area under the curve (AUC) of the oral glucose tolerance test for panel (a). (c) For the insulin tolerance test, the rats fasted for 2 h. Then, insulin (0.75 U/kg bw) was intraperitoneally injected, and the blood glucose concentration was measured as previously described. (d) The AUC of the insulin tolerance test for panel c. Each value represents the mean ± SD of three different mice (n = 3/group). Alphabet letters indicate intergroup comparisons. Different alphabets indicate statistically significant differences among groups (P<0.01). (e) Western blot was employed to detect ACSL1 protein in the adipose tissue. The image is a cropped blot and the full-length images of the western blot are provided in Supplementary Fig. S5a and b. Each value represents the mean ± SD of three different mice (n = 3/group). Alphabet letters indicate intergroup comparisons. Different alphabets indicate statistically significant differences among groups (P<0.01).